Our assays and tests

Some examples of assays and tests on 3D ExAdEx tissue models

ExAdEx’s R&D ex vivo platform offers customizable assays and analytical services to meet the specific needs of our partners. Our comprehensive suite of techniques spans molecular, functional, and metabolic activity assays, as well as advanced cell-based analysis and state-of-the-art 3D/4D tissue imaging systems for both scientific and commercial applications.

Our platform enables the testing of pharmacological compounds or active principles across multiple targets within a single study, including stem cells, mature adipocytes, extracellular matrix, vascularization, and lymphatic networks, among others. This multiparametric capability makes ExAdEx models ideal for multi-layered investigations and high-content analysis.

In collaboration with leading French academic partners, specialized technical platforms, and key opinion leaders (KOLs) in adipose tissue biology, we also offer the development of bespoke, on-demand models and assays. These innovative approaches allow for a deeper understanding of mechanisms of action within our pioneering 3D donor-derived native adipose tissue models.

FIRST-IN-HUMAN DATA GENERATION EX VIVO

FUNCTIONAL ASSAYS

Multiple native pathways preserved within the same 3D Donor-dervived Adipose Tissue ExAdEx models

Fat accumulation by glucose/lipids uptake assays and Glut4 expression.

Lipids release and Lipolysis by glycerol release assay.

Endocrine activity by secretome analysis via ELISA (Adiponectin, IL-6, IL-10, etc.) or multiplex assay.

Metabolic activity by XTT/MTT assay

Beige/Brown Adipose Tissue activity by batokine secretion analysis via ELISA

TRANSCRIPTOME ANALYSIS

Real-time quantitative gene expression (RT-qPCR) on different combination of genes at once

Stem cells and adipogenesis (adipogenic lineage markers)

Vascularization and Lymphatic drainage (CD31, Lyve-1, Prox1, etc.)

Thermogenesis and Beiging phenotype (UCP1)

Extracellular matrix remodeling (collagen, elastin, fibronectin, laminin, etc)

Fibrosis (cytokines, effectors)

Inflammation (cytokines, effectors, immune response)

3D IMAGING AND VIDEO

Showing compound effect on a 3D native adipose tissue environment                                                                 

Fibrosis by SHG confocal imaging and immunostaining (collagen, elastin, laminin, etc)

Inflammation by immunostaining (extracellular matrix alteration)

Effect on vascularization and lymphatic drainage by staining for vascular and lymphatic markers and 3D/4D video reconstruction

Lipolysis by adipocyte size measurement 

Quantitative analyses (available tools or tailored method development)

Thermogenic Beige/Brown Adipose Tissue Differentiation imaging (UCP1 immunostaining) as observed in clinical context

Image reconstruction and 3D/4D video generation

SAFETY AND COMPLEMENTARY
TESTING

Preliminary screening (if no previous information is available) or complementary testing for compounds and active ingredients

 

Targeting the safety dose through solubilisation assays and dose response study

Quantification of cell toxicity  by LDH activity

Metabolic activity of cells by XTT assay

Imaging of 3D morphology by Confocal 3D acquisitions

ADVANCED OR ON-DEMAND TECHNIQUES

Deeper knowledge of adipose tissue cell signature during differentiation and physio-pathological processes (obesity-like models, beiging models)

 

Cell proliferation by EdU staining

Progenitor and Adipocyte cell subtypes characterization by flow cytometry, confocal imaging, immunostaining and other tools

Development of tailored models (metabolic diseases, skin health, etc)

Assay and technique development (tailored imaging protocols, quantitative analysis tools, marker identification, etc)

SCIENTIFIC AND MARKETING VALUE

Bringing value to generated data through a combination of know-how and technology                                                                                                                 

Guidance in defining and tailoring R&D strategy thanks to KOLs in adipose tissue biology and adipose tissue research tools, among our co-founders 

Collaboration to scientific communication strategy and paper preparation

Marketing and communication assistance through articles, papers, presentation, 3D/4D videos for specific audiences

Identification of novel claims or mechanisms of actions with high differentiating value

GET IN TOUCH

We are happy to answer all your questions, discuss your needs and provide you with tailored solutions.
Please contact us and we will answer as soon as possible.
EXADEX-INNOV
Bioincubateur
Institut de Biologie Valrose
28 Avenue de Valombrose
06100 Nice (FRANCE)

Legal mentions

Copyright © 2022 ExAdEx-Innov All rights reserved