Our assays and tests

Some examples of assays and tests on 3D ExAdEx tissue models

ExAdEx’s R&D ex vivo platform offers assays and analytical services to meet the specific needs of our partners. Our comprehensive suite of techniques spans molecular, functional, and metabolic activity assays, as well as advanced cell-based analysis and state-of-the-art 3D and 4D tissue imaging systems (lightsheet, confocal microscopy, tomography) for both scientific and commercial applications.

Our platform enables the testing of compounds, active ingredients, natural extracts or products across multiple targets within a single study, including stem cells, connective cells, extracellular matrix, vascularization, and lymphatic networks, among others. This multiparametric capability makes ExAdEx models ideal for multi-layered investigations and high-content analysis.

In collaboration with leading French academic partners, specialized technical platforms, and key opinion leaders (KOLs), we also offer the development of bespoke, on-demand models and assays. These innovative approaches allow for a deeper understanding of mechanisms of action within our pioneering 3D donor-derived native tissue models.

ETHICAL. RELIABLE. INNOVATIVE.

Vascular and Lymphatic
3D Imaging
Facial Models
Fibro-Inflammation
Fat Burning and Slimming

VASCULAR AND LYMPHATIC MODELS

Lymphatic Network 

Lymphatic network markers by Real Time Quantitative PCR (RT-qPCR): Lymphangiogenesis, Lymphatic Function

3D Imaging and Quantitative parameters fro structure analysis: Network Reticulation, Fragmentation, Surface

Effect on lymphatic drainage through 3D and 4D video reconstruction

Vascular Network

Vascular network markers by Real Time Quantitative PCR (RT-qPCR): Angiogenesis, Vascular inhibition, Vascular Strengthening

3D Imaging and Quantitative parameters: Network Regularity, Diameter, Surface

Microcirculation and Blood Extravasation by ELISA, RT-qPCR or 3D Imaging

Effect on vascularization through 3D and 4D video reconstruction

FIBROSIS AND INFLAMMATION MODELS

Inflamm’Aging 

Markers linked to cellular senescence and inflammation by RT-qPCR and ELISA: cytokines, cell cycle markers by 

Mitochondrial activity (XTT analysis) for age-related metabolic dsyfunction

Extracellular matrix remodeling: collagen, elastin, fibronectin, laminin, etc.

Immunostaining and 3D imaging for Stem Cells depletion

Fibrosis

Fibrotic Collagen fiber deposition by Second Harmonic Generation (SHG) imaging

Fibrosis staining (PicroSiriusRed) and immunostaining for collagen fibers

Senescence-associated fibrosis markers by RT-qPCR and ELISA

CELLULITE AND SLIMMING MODELS

Slimming and Metabolic Models         

Fat-burning activity: lipolysis, adipocyte size reduction, thermogenesis, oxydative respiration

Metabolic activity: glucose metabolism, lipid metabolism by functional assays (glucose uptake, lipolysis, etc), RT-qPCR marker assessment and ELISA

Secreted hormones and cell effectors (adipokines) through ELISA

3D tissue structure and amelioration of cellulite phenotype: aqueous, fibrose, adipose

Image reconstruction, 3D and 4D video generation

SAFETY AND COMPLEMENTARY
TESTING

Preliminary screening or complementary testing for compounds and active ingredients

Targeting the safety dose through solubilisation assays and dose response study

Quantification of cell toxicity  by LDH activity

Normal metabolic activity of cells

Imaging of 3D morphology by Confocal 3D acquisitions

ADVANCED OR ON-DEMAND TECHNIQUES

Deeper knowledge of tissue cell networks interactions during physio-pathological processes 

Cell proliferation by EdU staining

Progenitor Cell subtypes characterization by flow cytometry, confocal imaging, immunostaining and other tools

Development of tailored models (skin health, metabolic conditions, etc)

Assay and technique development (tailored imaging protocols, quantitative analysis tools, marker identification, etc)

3D IMAGING VIDEO FOR SCIENTIFIC AND MARKETING VALUE

Bringing value to generated data through a combination of know-how and technology                                     

Guidance in defining and tailoring R&D strategy

Collaboration to scientific communication strategy and paper preparation

Marketing and communication assistance through articles, papers, presentation, 3D/4D videos for specific audiences

Identification of novel claims or mechanisms of actions with high differentiating value

GET IN TOUCH

We are happy to answer all your questions, discuss your needs and provide you with tailored solutions.
Please contact us and we will answer as soon as possible.
EXADEX-INNOV
Bioincubateur
Institut de Biologie Valrose
28 Avenue de Valombrose
06100 Nice (FRANCE)

Legal mentions

Copyright © 2022 ExAdEx-Innov All rights reserved

KOL on adipose tissue biology.

Research Director (DR1 INSERM).
Team Leader of « Stem Cells and Differentiation » Lab at Institut de Biologie Valrose (Nice). 

Label of Laboratoire d’Excellence by the French National Research Agency (Investissements pour le Futur, Labex Signalife program). 

Co-inventor of 5 patents

Expert in clinical applications of adipose tissue.

Plastic Reconstructive and Aesthetic Surgeon in Nice.

Member of the Medical Council of Alpes Maritimes region.

Expert for the French National Regulatory Agency ANSM for medical devices.

Certified by French Health Autority HAS (2020-2024).

PhD thesis (2018) on fat tissue and adipose tissue stem cells grafting.

Expert in cell therapy and immunology. 

Senior Lecturer (Maitre de conférences).

Responsible of the Cell and Gene Therapy Unity of Nice University Hospital (CHU de Nice).

Director of « Micoralis » research team at Université Côte d’Azur (Nice).

Founder and CEO of the biotech company Biointerférence (2003-2006).

Co-inventor of 5 patents.

Vincent is responsible of company strategy and technological innovation, thanks to his double skill set in science and deeptech startup development.

With a deep knowledge and vision about ExAdEx technology, Vincent has actively worked as a deeptech entrepreneur to push further the development of breakthrough innovative products around human adipose tissue, which lead him to create ExAdEx-Inov. 

In addition to his entrepreneurial skills, Vincent has a strong background in biology (PhD in Cellular and Molecular Biology from Université Côte d’Azur) and training in clinical research management (CRA training at the Faculty of Medicine of La Timone, Marseille). 

He is co-inventor of the 2 company’s patent families. 

Main areas of expertise: DeepTech entrepreneur; Bioengineering of human adipose tissue. 

Luigi is responsible of the management of company operations and business development strategy, thanks to his expertise in biotech startup development.

With a double competence and training in business and science (including an MBA from IAE Paris Sorbonne Business School and a PhD in Cytological Sciences from Rome University La Sapienza), Luigi has been managing business development and R&D projects in a preclinical CRO and a biotech startup company in previous years. 

He has been supporting ExAdEx-Innov project and business development since his position as an Entrepreneur in Residence for CNRS Innovation Tech Transfer Office in 2021.

Main areas of expertise: Biotech Startup Business Development; Project management