Our assays and tests

Some examples of assays and tests on ExAdEx 3D models

ExAdEx R&D ex vivo platform includes some tailored assays and analyses to fit your needs and claims substantiation goals with various techniques ranging from molecular to functional assays, metabolic activity, cell analysis as well as advanced 3D/4D tissue imaging tools for scientific or marketing purposes.

The effect of pharmacological compounds or active principles can be tested on different targets within the same study: Stem Cells, Mature Adipocytes, Extra-cellular Matrix, Vascularisation, Lymphatic network, etc). That’s the power of our ExAdEx models: a multiparametric tool for multiple layers of investigation.

Working in close collaboration with French academics partners and specialized technical platforms, as well as KOLs in adipose tissue biology, we can also develop tailored, on-demand models and new assays for a deeper comprehension of mechanisms of actions on the first 3D human adipose tissue models.

CLAIM SUBSTANTIATION AND EFFICACY

ExAdEx beige adipose tissue
metabolism, thermogenesis and beiging- exadex
inflammation-fibrosis-exadex
vascular-lymphatic-exadex
3D reconstruction

FUNCTIONAL ASSAYS

Multiple native pathways preserved within the same 3D Human Adipose Tissue ExAdEx models

Fat accumulation by glucose/lipids uptake assays and Glut4 expression.

Lipids release and Lipolysis by glycerol release assay.

Endocrine activity by secretome analysis via ELISA (Adiponectin, IL-6, IL-10, etc.) or multiplex assay.

Metabolic activity by XTT/MTT assay

Beige/Brown Adipose Tissue activity by batokine secretion analysis via ELISA

TRANSCRIPTOME ANALYSIS

Real-time quantitative gene expression (RT-qPCR) on different combination of genes at once

Stem cells and adipogenesis (adipogenic lineage markers)

Vascularization and Lymphatic drainage (CD31, Lyve-1, Prox1, etc.)

Thermogenesis and Beiging phenotype (UCP1)

Extracellular matrix remodeling (collagen, elastin, fibronectin, laminin, etc)

Fibrosis (cytokines, effectors)

Inflammation (cytokines, effectors, immune response)

3D IMAGING AND VIDEO

Showing compound effect on a 3D native adipose tissue environment                                                                 

Fibrosis by SHG confocal imaging and immunostaining (collagen, elastin, laminin, etc)

Inflammation by immunostaining (extracellular matrix alteration)

Effect on vascularization and lymphatic drainage by staining for vascular and lymphatic markers and 3D/4D video reconstruction

Lipolysis by adipocyte size measurement 

Quantitative analyses (available tools or tailored method development)

Thermogenic Beige/Brown Adipose Tissue Differentiation imaging (UCP1 immunostaining) as observed in clinical context

Image reconstruction and 3D/4D video generation

SAFETY AND COMPLEMENTARY
TESTING

Preliminary screening (if no previous information is available) or complementary testing for compounds and active ingredients

 

Targeting the safety dose through solubilisation assays and dose response study

Quantification of cell toxicity  by LDH activity

Metabolic activity of cells by XTT assay

Imaging of 3D morphology by Confocal 3D acquisitions

ADVANCED OR ON-DEMAND TECHNIQUES

Deeper knowledge of adipose tissue cell signature during differentiation and physio-pathological processes (obesity-like models, beiging models)

 

Cell proliferation by EdU staining

Progenitor and Adipocyte cell subtypes characterization by flow cytometry, confocal imaging, immunostaining and other tools

Development of tailored models (metabolic diseases, skin health, etc)

Assay and technique development (tailored imaging protocols, quantitative analysis tools, marker identification, etc)

SCIENTIFIC AND MARKETING VALUE

Bringing value to generated data through a combination of know-how and technology                                                                                                                 

Guidance in defining and tailoring R&D strategy thanks to KOLs in adipose tissue biology and adipose tissue research tools, among our co-founders 

Collaboration to scientific communication strategy and paper preparation

Marketing and communication assistance through articles, papers, presentation, 3D/4D videos for specific audiences

Identification of novel claims or mechanisms of actions with high differentiating value

Get in touch

We are happy to answer all your questions, discuss your needs and provide you with tailored solutions in the human adipose tissue field.
Please contact us and we will answer as soon as possible.
EXADEX-INNOV
Bioincubateur
Institut de Biologie Valrose
27 Rue Professeur Delvalle
06000 NICE – FRANCE

Legal mentions

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KOL on adipose tissue biology.

Research Director (DR1 INSERM).
Team Leader of « Stem Cells and Differentiation » Lab at Institut de Biologie Valrose (Nice). 

Label of Laboratoire d’Excellence by the French National Research Agency (Investissements pour le Futur, Labex Signalife program). 

Co-inventor of 5 patents

Expert in clinical applications of adipose tissue.

Plastic Reconstructive and Aesthetic Surgeon in Nice.

Member of the Medical Council of Alpes Maritimes region.

Expert for the French National Regulatory Agency ANSM for medical devices.

Certified by French Health Autority HAS (2020-2024).

PhD thesis (2018) on fat tissue and adipose tissue stem cells grafting.

Expert in cell therapy and immunology. 

Senior Lecturer (Maitre de conférences).

Responsible of the Cell and Gene Therapy Unity of Nice University Hospital (CHU de Nice).

Director of « Micoralis » research team at Université Côte d’Azur (Nice).

Founder and CEO of the biotech company Biointerférence (2003-2006).

Co-inventor of 5 patents.

Vincent is responsible of company strategy and technological innovation, thanks to his double skill set in science and deeptech startup development.

With a deep knowledge and vision about ExAdEx technology, Vincent has actively worked as a deeptech entrepreneur to push further the development of breakthrough innovative products around human adipose tissue, which lead him to create ExAdEx-Inov. 

In addition to his entrepreneurial skills, Vincent has a strong background in biology (PhD in Cellular and Molecular Biology from Université Côte d’Azur) and training in clinical research management (CRA training at the Faculty of Medicine of La Timone, Marseille). 

He is co-inventor of the 2 company’s patent families. 

Main areas of expertise: DeepTech entrepreneur; Bioengineering of human adipose tissue. 

Luigi is responsible of the management of company operations and business development strategy, thanks to his expertise in biotech startup development.

With a double competence and training in business and science (including an MBA from IAE Paris Sorbonne Business School and a PhD in Cytological Sciences from Rome University La Sapienza), Luigi has been managing business development and R&D projects in a preclinical CRO and a biotech startup company in previous years. 

He has been supporting ExAdEx-Innov project and business development since his position as an Entrepreneur in Residence for CNRS Innovation Tech Transfer Office in 2021.

Main areas of expertise: Biotech Startup Business Development; Project management